In SMA mice, quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) revealed significant alterations in NF transcript and protein levels (both NfH and NfL) in spinal cord and blood across disease stages and phenotypes (Spicer et al. [43]). The gene discussed is NFASC; the disease is proximal spinal muscular atrophy.