In the co-culture model established with Jurkat cells pre-stimulated for 72 h using 10 μg/mL of PHA-M, 1 μg/mL of recombinant human PD-1 protein, and breast cancer cells, PD-1/PD-L1 interaction was targeted using BMS-1166 at 2 μM and 20 μM and Human PD-L1 Inhibitor IV at 1 μg/mL and 10 μg/mL. Here, PDCD1 is linked to breast carcinoma.