The lymphocytes associated with the tumor immune microenvironment (TIME) are represented by all major subpopulations, including: CD20+ B-cells, CD3+CD4+ helper T-cells, CD3+CD8+ cytotoxic T-cells and the forkhead box P3-positive (FOXP3+) regulatory T-cells (Tregs) [54,59]. The gene discussed is CD8A; the disease is neoplasm.