If we can investigate the differentially expressed gene (DEG) regarding mast cell tumors, such as CD 117, Beclin-1, and Ki-67 in the primary lymph node tissue, and visualize the spatial RNA transcription at the single-cell level (Figure 3) using 10× visium and Seurat package in R, it will provide a deeper understanding of the development of mast cell tumors and the reason for their rarity. This evidence concerns the gene MKI67 and mast cell neoplasm.