In order to gain an unbiased, thorough understanding of the metabolic activity within T-cell subpopulations in the TME, we conducted Ucell analysis, focusing on the pentose phosphate, glycolysis, and oxidative phosphorylation metabolic processes for CD4+ T cells, CD8+ T cells, Treg cells, and NK cells in the tumor and in normal tissues (Figure 3F). This evidence concerns the gene CD8A and neoplasm.