Significant fluorescent signals were observed within the tumor sites of the U251/NC, U251/PEG3-FTH1, and U251/PEG3-bFGF2 5′UTR-FTH1-WPRE groups (Figure 4A), indicating successful intratumoral viral transduction and validating the progression to subsequent experiments. The gene discussed is FTH1; the disease is neoplasm.