Additionally, hyperglycemia/obesity was strongly associated with significant activation of the MGO-AGE-RAGE pathway in bladder tissues, as indicated by the elevated levels of total AGEs, MG-H1, and Glo1 activity, along with a decreased antioxidant system, as evidenced here by the reduced enzyme activities of GR, GPX, and SOD. This evidence concerns the gene GSR and obesity due to melanocortin 4 receptor deficiency.