TLR4 and metabolic dysfunction-associated steatohepatitis: In a study by Depner et al. [76], 16 weeks of omega-3 supplementation in an Ldlr−/− mouse model of Western diet-induced nonalcoholic steatohepatitis (NASH) significantly reduced hepatic pro-inflammatory markers, including TNF-α, TLR4, and IL-1β.