In the in vitro study, cardiac hypertrophy induced with 50 μM phenylephrine in neonatal rat cardiomyocytes and treated with 5 μM TCA, TCA was found to suppress cardiac hypertrophy by decreasing the phosphorylation and nuclear localization of calcium/calmodulin-dependent protein kinase II (CaMKII) and extracellular signal-related kinase (ERK) in neonatal rat cardiomyocytes, while it blocked the hyperphosphorylation of ryanodine receptor type 2 and phospholamban, as well as restoring calcium handling and sarcomere shortening in adult mice cardiomyocytes. The gene discussed is RYR2; the disease is cardiac hypertrophy.