Our findings revealed many somatic mutated candidate biomarkers and driver genes transcriptionally associated with CRC, including CDH3, KRT80, ETV4, ESM1, FOXQ1, WNT2, CLDN1, AJUBA, NFE2L3, BEST4, CPNE7, INHBA, MTHFD1L, MMP7, PEX26, KLK6, TRIB3, KRT23, CEMIP, TRIP13, PHLPP2, SLCO4A1, MDFI, NOTUM, ENC1, VWA2, and LARGE2, from tumors versus controls, which have been experimentally confirmed to be directly involved in CRC [28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54]. Here, MTHFD1L is linked to colorectal carcinoma.