Medical management of endometriosis may alter tissue architecture and staining patterns; to mitigate this, we performed comprehensive H&E staining and selected only those slides containing both stromal and epithelial components for VDR immunohistochemistry, ensuring consistent assessment of VDR expression to investigate how vitamin D functions through VDR, enabling active vitamin D to engage in cellular anti-inflammatory and immunomodulatory pathways. Here, VDR is linked to endometriosis.