Then, we screened the nine locally established ESCC cell lines (KYSE-30, KYSE-70, KYSE-140, KYSE-150, KYSE-180, KYSE-270, KYSE-410, KYSE-450, and KYSE-510) for their EGFR expression level using qPCR (Figure 1B), and we established that KYSE-30 and KYSE-150 have the highest and lowest levels of EGFR mRNA, respectively. The gene discussed is EGFR; the disease is esophageal squamous cell carcinoma.