The precise dissection of CX3CR1/CX3CL1 spatiotemporal dynamics requires advanced technologies: single-cell RNA sequencing (scRNA-seq) reveals molecular heterogeneity and temporal evolution of CX3CR1-expressing myeloid subsets post-stroke [20,21,22,23], while spatial transcriptomics captures astrocyte-derived CX3CL1 enrichment gradients within critical repair regions [24,25]. The gene discussed is CX3CL1; the disease is Stroke.