FLI1 and sarcoma: To address that, we crossed four datasets comprising: EWS::FLI1 transcriptionally regulated genes (A673/TR/shEF; 36 h DOX treatment) [41], differentially expressed genes between EwS and non-EwS sarcoma cell lines [42], and two R-loop interactomes [17, 43] (Supplementary Fig. 3A).