To validate the expression of B7-H3 in CRC cell lines, we detected B7-H3 using flow cytometry in five commonly used human CRC cell lines (SW620, HCT116, HT29, LS174T and LoVo), and found a high expression of B7-H3 in all tested cells (Fig. 2a). This evidence concerns the gene CD276 and colorectal carcinoma.