We instead concentrated on combinations of alterations associated with liver cancer, including overexpression of oncogenic drivers (Myc, mutant Ctnnb1 (mtCtnnb1), Vegfa and NICD) and silencing of tumour suppressors (Trp53, Pten and Kmt2c) with short hairpin RNA (shRNA) alongside a frequently used Renilla luciferase (shRen)-targeting control construct13,22. The gene discussed is VEGFA; the disease is liver cancer.