Glioblastoma cell–derived exosomes carrying thesemarkers were isolated from both the blood and cerebrospinal fluidof a mouse model, possibly representing a minimally invasive approachfor diagnosing glioblastoma, reducing the need for surgical biopsiesand enabling easier and more frequent monitoring of the disease. CD44 and CD133 were sensitively detected inimmunocaptured glioblastoma cell-derived exosomes, highlighting theirpotential as diagnostic markers. The gene discussed is PROM1; the disease is glioblastoma.