This technique involves theproteomic fingerprinting of intact nanoscale EVs from clinical samples.Through quantitative analysis of EV fingerprints using MALDI-TOF MS,specific proteins such as SAA proteins (SAA1-1, desR-SAA1-2, SAA2,SAA1-2) were identified with AUCs ranging from 0.92 to 0.97, enablingthe detection of acute pancreatitis within 30 min. Here, SAA2 is linked to acute pancreatitis.