Electrochemical sensing strategies integratedwith aptamers, DNAnanomachines, rolling circle amplification (RCA), and label-free andhomogeneous electrochemical techniques have shown promise for tumor-derivedexosome detection via simultaneously targeting exosomal MUC1 and PD-L1.This approach was incorporated into a separation-free electrochemicaldetection assay, and the results strongly correlated with findingsfrom computerized tomography and pathological analyses, demonstrating100% specificity, 92% sensitivity, and an overall accuracy of 94.6%,with an AUC of 0.97. This evidence concerns the gene CD274 and neoplasm.