In our study, we found that pretreatment with NBQX inhibited LS and was associated with decreased expressions of p‐NR1 (Ser897) and NR2B (Tyr1472) subunits, as well as D1R levels These findings are in agreement with a previous observation that D1R antagonists decreased the expressions of p‐NR1 (Ser897) and p‐NR2B (Tyr1472) subunits in the model of tumour cell implantation induced bone cancer pain in rats [38]. This evidence concerns the gene GRIN2B and bone cancer.