The results showed that L. rhamnosus Glory LG12 could promote the secretion of inhibitory transmitters such as MTL, Gas, SP, ACH etc., and then enhance the intestinal muscle motility of mice, which effectively improved the intestinal peristalsis of constipated mice.; Also, L. rhamnosus Glory LG12 reduces the concentrations of the inhibitory transmitters VIP, SS, PYY and 5-HT, and reduces the contraction of small intestinal smooth muscle, thereby treating constipation. Here, HTR5A is linked to constipation disorder.