The assay can detect key drug resistance markers in the genescrt,dhfr,dhps, andmdr1; mutations in the propeller domain ofkelch13 that are associated with artemisinin partial resistance; diversity in thecsp vaccine target and the polymorphic surface antigenmsp2 – which may be used as an approximate indicator of multiplicity of infection; and uses the 18S rRNA gene to identifyPlasmodium species. Here, DHFR is linked to infection.