Figure 7 shows the flow cytometric profile of a stage IV salivary gland cancer patient, from which CTC were collected. A total of 603 ng of DNA was extracted from the 46,423 cells in the CTC fraction of this patient. Exome sequencing of the CD45+Vimentin- cells (normal hematopoietic fraction) and the CD45-Vimentin+ cells (CTC fraction) were carried out, and 108 neoantigens were identified using the Ancer platform in the patient with salivary gland cancer. The top ranked 30 neoantigens are shown in Table 3 similar to Table 2. This evidence concerns the gene VIM and salivary gland cancer.