For instance, for brain cancer and glioblastoma models, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) or insulin-like growth factor (IGF) are used along with neurobasal medium supplemented with B27 or N2 to replicate brain tumor signaling and support neural cell growth (Figure 5; 51, 53),. The gene discussed is EGF; the disease is brain neoplasm.