Using a mouse model of AKI, we demonstrated that cisplatin‐induced obvious inflammation in the kidney, evidenced by increased macrophage and neutrophil infiltration, elevated expression of pro‐inflammatory cytokines, including interleukin‐1 beta (IL‐1β), IL‐6, C‐X‐C motif chemokine ligand (CXCL) 1, 2, 5, 20, and C‐C motif chemokine ligand (CCL) 2, and activation of the toll‐like receptor 4 (Tlr4)/nuclear factor‐kappa B (NF‐κB) pathway. Here, NFKB1 is linked to acute kidney injury.