The findings suggest that R. tripartita extracts induce cell cycle arrest and apoptosis via the PI3K/AKT/mTOR signaling pathway by reducing phosphorylation of mTORC1 substrate S6Ser2040‐244 and mTORC2 downstream target AKT‐Ser473 in acute monocytic leukemia THP‐1 cells. The gene discussed is PIK3CA; the disease is acute monocytic leukemia.