CD274 and glioma: To demonstrate that TRAF3IP3 was indeed knocked down when transfecting specific siRNAs into the glioma cells, we performed three biological replicates in a new round of transfection in U251, finding that TRAF3IP3 was knocked down and PDL1 was downregulated (Figure 7C), which was validated in our in‐house cohort (Figure 7D).