To demonstrate that TRAF3IP3 was indeed knocked down when transfecting specific siRNAs into the glioma cells, we performed three biological replicates in a new round of transfection in U251, finding that TRAF3IP3 was knocked down and PDL1 was downregulated (Figure 7C), which was validated in our in‐house cohort (Figure 7D). Here, CD274 is linked to central nervous system cancer.