To explore the relationship between TTPAL and ESCC progression, we measured TTPAL protein levels in ESCC cells and selected four cell lines (KYSE180, KYSE30, KYSE510, and KYSE150) with different TTPAL expression levels for further experiments (Supplementary Fig. 1A, 1B). The gene discussed is TTPAL; the disease is esophageal squamous cell carcinoma.