As shown in Supplementary Fig. 3A and 3B, shAKT abolishes the decrease of I-kBα observed in Fig. 3A and B. We also further used these transfected cells to perform the cell nuclear and cytoplasmic fractionation, showing IGF2 could not enhance the translocation of NF-κB into nucleus in HD cells under the shAKT condition (Supplementary Fig. 3C, lane 5 and lane 6). This evidence concerns the gene NFKBIA and Huntington disease.