However, in primary mouse cortical neurons, TMEM106B deficiency has been shown to cause impairment in lysosomal acidification [137], although TMEM106B overexpression has mixed results with some showing an impairment in lysosomal acidification in HeLa cells [136, 143] and others reporting enhanced acidification in HEK293T cells and murine and human lung cancer cell lines [132, 148]. The gene discussed is TMEM106B; the disease is lung cancer.