Unsurprisingly, GNS was significantly elevated in the carrier group relative to the MPS IIID group and CD68 was significantly elevated in the MPS IIID group relative to the carrier group (Figs 3B and 3E), confirming our disease phenotype is due to appropriate gene knockout and correlating with our immunofluorescence (IF) data (see below). This evidence concerns the gene GNS and mucopolysaccharidosis type 3D.