This aligns with previous in vivo studiesthat mention CD44+/CD24– cells are localized at the tumor boundary(known for high perfusion rates), while ALDH + cells reside in thetumor core (known for an acidic microenvironment)., It is known that B–CSCs exhibit plasticity, transitioningbetween E-CSCs and M-CSCs, and the TMEcould potentially influence this transition. This evidence concerns the gene CD24 and neoplasm.