These results indicate that demethylation guided by the pdCas9-C-Tet1-SgRNA 2.0 system on the AKT1 promoter can reactivate the expression of AKT1 and AKT1/mTOR signaling pathway-related proteins by reducing the AKT1 promoter’s methylation level and promoting the recovery milk protein expression in BMMECs, thereby alleviating the symptoms of mastitis [70]. This evidence concerns the gene MTOR and mastitis.