Our investigation used a PCR-DNA-sequencing procedure to illustrate the difference in immunological (IL-1α, IL1B, IL6, TNF-α, IL10, and IFN-γ) and antioxidant (PRDX6, ATG7, NDUFS6, and NOX4) genes in calves with pneumonia and healthy calves. The gene discussed is NDUFS6; the disease is susceptibility to pneumonia measurement.