To confirm the attenuation of the FLT3 pathway in AML cells, we investigated the effects of compound 5-3 on the FLT3-mediated signaling pathway in BaF3-FLT3-ITD (Figure 2A,B) and MV4-11 (Figure 2C,D) cells. This evidence concerns the gene FLT3 and acute myeloid leukemia.