Clinical trial (NCT06042842) aims to (1) evaluate the clinical relevance of plasma circRNAs (hsa_circ_0004001) as a noninvasive diagnostic biomarker for HCC; (2) determine the relation of circRNAs (hsa_circ_0004001) to HCC staging; (3) compare between circRNAs (hsa_circ_0004001) and the routine marker (AFP) as biomarkers for HCC diagnosis. The gene discussed is AFP; the disease is hepatocellular carcinoma.