In our work, we used IL-2 (300 units/mL) and IL-15 (7 ng/mL) for 1 h to stimulate NK cell activation/expansion, and then used IL-15 (50 ng/mL) or TGFβ (5 ng/mL) for the ex-periment with 3D models of BC. The gene discussed is IL15; the disease is breast cancer.