CD40LG and Zika virus infectious disease: For example, Tsai et al. (2017) [39] developed an anti-DENV-1 NS1 IgG ELISA and demonstrated its ability, in combination with anti-ZIKV NS1 IgM and IgG ELISAs, to distinguish primary ZIKV infections from ZIKV with prior DENV exposure and secondary DENV infections [39].