Among the three RBPs that influence REST expression, we next focused on FUS in order to further investigate the molecular mechanism of REST mRNA regulation, given that depletion of FUS attenuated transcriptional regulation of UNC13A to a greater extent than did that of the other RBPs (Figs. 2G and 4F; Appendix Fig. S3E) and that FUS is more frequently mutated in individuals with ALS compared with the other RBP genes (Akiyama et al, 2016; Renton et al, 2014). This evidence concerns the gene UNC13A and amyotrophic lateral sclerosis.