To investigate the BANF1 interactome in the context of ASFV infection and DNA binding, we expressed the BANF1-GFP fusion protein in WSL cells under the four following experimental conditions: (i) absence of infection and benzonase treatment (mock-infected, no treatment), (ii) absence of infection and presence of benzonase treatment (mock-infected, benzonase treatment), (iii) presence of ASFV infection and absence of benzonase treatment (ASFV-infected, no treatment), and (iv) presence of ASFV infection and benzonase treatment (ASFV-infected, benzonase treatment). The gene discussed is BANF1; the disease is infection.