Combined with our immunocytochemistry results (Fig. 3) and tethering experiments (Supplementary Fig. S4A), this suggests that while Nsp2 recruits GIGYF2 near DMVs to increase GIGYF2’s local concentration during infection, Nsp2 is not required for GIGYF2 activity per se under uninfected conditions. Here, GIGYF2 is linked to infection.