The number of clinical samples from 50 APS and 50 non-APS patients was relatively small to convert diagnostic thresholds in high precision, especially for aPL assays of BioPlex® APLS aCL IgG/IgM and QUANTA Lite® aβ2GPI IgM, in which a large proportion of samples had values below detection limits. Here, ACLY is linked to autoimmune polyendocrinopathy.