CD300A and renal fibrosis: To further analyze the CD300a involvement in the development of renal fibrosis, we used a 2-step unilateral IRI (uIRI) model (23), in which the left renal artery was clamped for 20 minutes (longer than for biIRI) and then reperfused, followed by removal of the right kidney on day 14 after uIRI (Supplemental Figure 2D), in Cd300afl/fl and Cd300afl/flLyz2-Cre mice.