(ii)Long-term persistent HBV replication and intermittent HBV activation caused by PSinjection are essential for CHB-LF progression; pAAV-HBV1.2 and PS injectionsestablish and sustain a microenvironment in the liver by driving functionalexhaustion of CD8+ T cells and reducing anti-viral antibody production.(iii) LF progression in model mice was mainly triggered by liver injury inducedafter each PS injection, with HBV itself being a necessary factor in thisprogression. The gene discussed is CD8A; the disease is Lassa fever.