Next, wild-type (WT) control mice (Anlnfl/fl) and Anln KO mice (Alb−/+; Anlnfl/fl) cohorts were started on the chronic MASH to HCC model using WD and high-sugar water, as well as weekly CCl4 injections for 32 weeks (Fig. 6B). Here, ANLN is linked to hepatocellular carcinoma.