To investigate the potential role of PDLIM3 in the invasive properties of GSCs, we performed 3D invasion assays utilizing invasive GSCs neurospheres obtained from GBM biopsy samples, in which PDLIM3 was knocked down using a specific siRNA si-PDLIM3(5), validated for its efficiency to inhibit its expression in the GBM neurospheres compared to a scramble control (figure 1G). This evidence concerns the gene PDLIM3 and glioblastoma.