To assess a potential off-target effect of the PDLIM3 siRNA, we used a second siRNA, si-PDLIM3(6) and showed in GBM neurospheres, a high inhibition of PDLIM3 expression (figure 1G) as well as similar results in 3D invasion assays (figure 1I). The gene discussed is PDLIM3; the disease is glioblastoma.