Upon analysis of gene expression in tissue from 4 control brainstems and 12 tumor samples (samples identified in Table S5), we found that gene CD276 (B7-H3) was significantly overexpressed in tumors, compared to brainstem controls, while CD274 (PD-L1), CD273 (PD-L2) and CD152 (CTLA-4) were not (Figure 2A). Here, CD274 is linked to neoplasm.