To gain an insight into the molecular pathological basis for NASH caused by Nrf1-/- in livers, further experiments revealed that the Nrf1-deficient hepatocytes acquired for increased susceptibility to oxidative stress and relevant damages, along with down-regulation of some ARE-battery genes (e.g. Gstm3, Gstm6 and Gstp2) but up-regulation of Cyp4A genes 261. This evidence concerns the gene NRF1 and metabolic dysfunction-associated steatohepatitis.