Rather, no significant changes in those prototypic Nrf2-target genes (e.g., Gclc, Gclm, Gss, Nqo1) were observed upon acute hepatic loss of Nrf1 in the 3-methylcholanthrene- inducible KO mice (Nrf1flox/flox:Cyp1A1-Cre), which also led to profound NASH, but without obvious oxidative stress 346. This evidence concerns the gene NRF1 and metabolic dysfunction-associated steatohepatitis.