ANPEP and Familial prostate cancer: Their antiproliferative activity was observed against THP-1 monocytic leukemia cells expressing APN, with IC50 values of 66.05 μM and 70.18 μM; against MCF-7 breast carcinoma cells, also expressing APN, with IC50 values of 39.94 μM and 75.47 μM, respectively; but showed minimal activity against DU-145 prostate cancer cells lacking APN expression, with IC50 values > 100 μM for both compounds.