Many of the genes with the highest shape disruption (e.g., HHEX, HOXB2, Additional file 1: Fig. S8b) arise early in AML, around the initiation of NPM1 mutation, and subsequently drop to similar expression levels in advanced blasts, highlighting the importance of enriching our data with early leukemic progenitors to detect such initial events. The gene discussed is NPM1; the disease is acute myeloid leukemia.