RUNX1 and acute myeloid leukemia: To determine whether dysregulated TF patterns can generalize, we compared the top disrupted TFs of all 3 AML patients (Additional file 1: Fig. S10) and observed strong similarity in the combined disruption score across patients, with key TF genes including HOXB3, HOXA3, and GATA1 among the top 20 disrupted TFs in AML1 and AML2 (Figs. 6c, d and S10a).