The similar patterns of key developmental markers CD34 [65], AVP (stemness), PABPC1 (protein synthesis in HSC differentiation), and LYZ (myeloid differentiation) in aligned segments of the trajectories defined in the integration of AML and healthy (Fig. 5b), as well as the patterns of key genes in the combination of AML patients across the cohort (Additional file 1: Fig. S3c), confirm that the inferred pseudotime is comparable between datasets. Here, AVP is linked to acute myeloid leukemia.