We activated CD45.1+ or CD45.2+ OT-I CD8+ T cells in 0.1 (control) or 0.03 mM Met for 30 min, restored Met to 0.1 mM for 24 h, and mixed them at a ratio of 1:1 before transferring them into B16-OVA tumor-bearing Rag1−/− mice (Fig. 2a). This evidence concerns the gene CD8A and neoplasm.