Importantly, compared with HFD-treated control mice, those with macrophage-specific EP3α overexpression (Rosa-EP3α/LysMCre) demonstrated significant amelioration in HFD-induced weight gain (Fig. 4A), improvement in glucose tolerance (Fig. 4B,C), alleviation in insulin resistance (Fig. 4D,E; Appendix Fig. S9C,D), decrease in white adipose tissue mass (Fig. 4F), reduction in adipocyte size (Fig. 4G–K), and downregulation in the expression of fatty acid synthesis and adipogenesis genes in adipose tissues (Fig. 4L–P). The gene discussed is EDDM3A; the disease is Insulin resistance.